Difference between revisions of "Part:BBa K3195003"
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This Cathespin B from Branchiostoma belcheri tsingtauense is originally a kind of hydrolase in lysosome.In our project,it has been used to biodegrade microcystin,a kind of biotoxin synthesized by cyanobacteria.
 
This Cathespin B from Branchiostoma belcheri tsingtauense is originally a kind of hydrolase in lysosome.In our project,it has been used to biodegrade microcystin,a kind of biotoxin synthesized by cyanobacteria.
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===Usage and Biology===
 
===Usage and Biology===
 
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Our BioBrick encodes a laccase from the fungus <i>Trametes versicolor</i> (PDB: 1GYC). Laccases are a class of multi-copper oxidases that fungi use to degrade lignin and be able to grow on wood [2]. Lignin is composed of a cluster of multiple phenolic groups, which are oxidized by laccases. With their natural affinity for phenolic-like structures, laccases have also been shown to degrade a large range of other phenolic compounds. For example, many reports show that laccases can be used to degrade pharmaceuticals - including estrogens, painkillers and antibiotics [3].
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Revision as of 10:08, 5 October 2019


His-tagged Cathepsin B

This Cathespin B from Branchiostoma belcheri tsingtauense is originally a kind of hydrolase in lysosome.In our project,it has been used to biodegrade microcystin,a kind of biotoxin synthesized by cyanobacteria.

Usage and Biology


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 369
    Illegal XhoI site found at 958
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 463
  • 1000
    COMPATIBLE WITH RFC[1000]