Difference between revisions of "Part:BBa K3195003"
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Our BioBrick encodes a laccase from the fungus <i>Trametes versicolor</i> (PDB: 1GYC). Laccases are a class of multi-copper oxidases that fungi use to degrade lignin and be able to grow on wood [2]. Lignin is composed of a cluster of multiple phenolic groups, which are oxidized by laccases. With their natural affinity for phenolic-like structures, laccases have also been shown to degrade a large range of other phenolic compounds. For example, many reports show that laccases can be used to degrade pharmaceuticals - including estrogens, painkillers and antibiotics [3]. | Our BioBrick encodes a laccase from the fungus <i>Trametes versicolor</i> (PDB: 1GYC). Laccases are a class of multi-copper oxidases that fungi use to degrade lignin and be able to grow on wood [2]. Lignin is composed of a cluster of multiple phenolic groups, which are oxidized by laccases. With their natural affinity for phenolic-like structures, laccases have also been shown to degrade a large range of other phenolic compounds. For example, many reports show that laccases can be used to degrade pharmaceuticals - including estrogens, painkillers and antibiotics [3]. | ||
Revision as of 10:04, 5 October 2019
His-tagged Cathepsin B
This Cathespin B from Branchiostoma belcheri tsingtauense is originally a kind of hydrolase in lysosome.In our project,it has been used to biodegrade microcystin,a kind of biotoxin synthesized by cyanobacteria.
Usage and Biology
Our BioBrick encodes a laccase from the fungus Trametes versicolor (PDB: 1GYC). Laccases are a class of multi-copper oxidases that fungi use to degrade lignin and be able to grow on wood [2]. Lignin is composed of a cluster of multiple phenolic groups, which are oxidized by laccases. With their natural affinity for phenolic-like structures, laccases have also been shown to degrade a large range of other phenolic compounds. For example, many reports show that laccases can be used to degrade pharmaceuticals - including estrogens, painkillers and antibiotics [3].
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 369
Illegal XhoI site found at 958 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 463
- 1000COMPATIBLE WITH RFC[1000]