Difference between revisions of "Part:BBa K3221202:Design"
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===Design Notes=== | ===Design Notes=== | ||
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===Source=== | ===Source=== | ||
Revision as of 14:54, 4 October 2019
pMET3-yeGFP-CYC1 terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 302
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 399
Illegal BsaI.rc site found at 1144
Design Notes
We designed this part to fit the RFC10.
Source
We get the pMET3 from the BY4742 genomic, and the plasmid with yeGFP from the Team Peking. The CYC1 terminator is initially on the pYES2.