Difference between revisions of "Part:BBa K2938000:Design"
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
None
+
Designed by our lab prior to the competition with a single point mutation to slow down initial translation initiation rates.
  
  
Line 19: Line 19:
 
Schlesinger, Orr, et al. "Tuning of recombinant protein expression in Escherichia coli by manipulating transcription, translation initiation rates, and incorporation of noncanonical amino acids." ACS synthetic biology 6.6 (2017): 1076-1085.
 
Schlesinger, Orr, et al. "Tuning of recombinant protein expression in Escherichia coli by manipulating transcription, translation initiation rates, and incorporation of noncanonical amino acids." ACS synthetic biology 6.6 (2017): 1076-1085.
 
https://www.biorxiv.org/content/10.1101/100982v1.full
 
https://www.biorxiv.org/content/10.1101/100982v1.full
 +
 +
Shin, Jonghyeon, and Vincent Noireaux. "Efficient cell-free expression with the endogenous E. Coli RNA polymerase and sigma factor 70." Journal of biological engineering 4.1 (2010): 8.

Latest revision as of 13:17, 4 October 2019


Attenuated pBEST-PR promoter after point mutation


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 56
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed by our lab prior to the competition with a single point mutation to slow down initial translation initiation rates.


Source

This promoter was already in use in our lab.


References

Schlesinger, Orr, et al. "Tuning of recombinant protein expression in Escherichia coli by manipulating transcription, translation initiation rates, and incorporation of noncanonical amino acids." ACS synthetic biology 6.6 (2017): 1076-1085. https://www.biorxiv.org/content/10.1101/100982v1.full

Shin, Jonghyeon, and Vincent Noireaux. "Efficient cell-free expression with the endogenous E. Coli RNA polymerase and sigma factor 70." Journal of biological engineering 4.1 (2010): 8.