Difference between revisions of "Part:BBa K2996001:Design"

(→‎Design Notes)
Line 16: Line 16:
 
===References===
 
===References===
 
1.Sheth R U , Yim S S , Wu F L , et al. Multiplex recording of cellular events over time on CRISPR biological tape[J]. Science, 2017:eaao0958.
 
1.Sheth R U , Yim S S , Wu F L , et al. Multiplex recording of cellular events over time on CRISPR biological tape[J]. Science, 2017:eaao0958.
 +
2.Arslan Z , Hermanns V , Wurm R , et al. Detection and characterization of spacer integration intermediates in type I-E CRISPR-Cas system[J]. Nucleic Acids Research, 2014, 42(12):7884-7893.

Revision as of 10:59, 4 October 2019


cas1-cas2 complex


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 116
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 837
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

cas1 and cas2 indeed are seperated by one base pair, as it is in E.coli genome.

Source

The pRec plasmid containing cas1-cas2 is donated from Harris H. Wang from Columbia University. The cas1-cas2 cassette is amplified from NEB 10-beta. They are the only two Cas proteins found in all CRISPR–Cas systems, so cas1-cas2 are pretty conserved.

References

1.Sheth R U , Yim S S , Wu F L , et al. Multiplex recording of cellular events over time on CRISPR biological tape[J]. Science, 2017:eaao0958. 2.Arslan Z , Hermanns V , Wurm R , et al. Detection and characterization of spacer integration intermediates in type I-E CRISPR-Cas system[J]. Nucleic Acids Research, 2014, 42(12):7884-7893.