Difference between revisions of "Part:BBa K3221008:Design"
(→Design Notes) |
(→Source) |
||
| (One intermediate revision by the same user not shown) | |||
| Line 10: | Line 10: | ||
===Source=== | ===Source=== | ||
| − | + | pGAL1 and tCYC1 is on pYES2 initially. And we get HisG-only by PCR from CNS3(BBa_K3221001). | |
| − | + | ||
===References=== | ===References=== | ||
Latest revision as of 09:51, 4 October 2019
pGAL1-HisG only-tCYC1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 150
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We use double enzymes restriction of HindIII and XbaI before adapter ligation.
Source
pGAL1 and tCYC1 is on pYES2 initially. And we get HisG-only by PCR from CNS3(BBa_K3221001).