Difference between revisions of "Part:BBa K3221008:Design"
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===Source=== | ===Source=== | ||
| − | + | pGAL1 and tCYC1 is on pYES2 initially. And we get yeGFP by gene synthesis. | |
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===References=== | ===References=== | ||
Revision as of 09:50, 4 October 2019
pGAL1-HisG only-tCYC1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 150
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We use double enzymes restriction of HindIII and XbaI before adapter ligation.
Source
pGAL1 and tCYC1 is on pYES2 initially. And we get yeGFP by gene synthesis.