Difference between revisions of "Part:BBa K3221008:Design"

 
(Design Notes)
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===Design Notes===
 
===Design Notes===
pGAL1 and tCYC1 is on pYES2 initially. And we get yeGFP by gene synthesis.
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We use double enzymes restriction of HindIII and XbaI before adapter ligation.
 
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===Source===
 
===Source===

Revision as of 09:49, 4 October 2019


pGAL1-HisG only-tCYC1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use double enzymes restriction of HindIII and XbaI before adapter ligation.

Source

We use double enzymes restriction of HindIII and XbaI before adapter ligation.

References