Difference between revisions of "Part:BBa K3022002:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | PCR-amplified CFppk1 was digested with restriction enzymes Kpn I and Hind III, after which it was insert into corresponding multiple cloning sties of the broad-host-range expressing vector pBBR1MCS2. | |
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===References=== | ===References=== | ||
| + | Wang X , Wang X , Hui K , et al. Highly Effective Polyphosphate Synthesis, Phosphate Removal and Concentration Using Engineered Environmental Bacteria Based on a Simple Solo Medium-copy Plasmid Strategy[J]. Environmental Science & Technology, 2017:acs.est.7b04532. | ||
Latest revision as of 09:27, 3 October 2019
ppk1 in Citrobacter freundii ATCC 8090
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
PCR-amplified CFppk1 was digested with restriction enzymes Kpn I and Hind III, after which it was insert into corresponding multiple cloning sties of the broad-host-range expressing vector pBBR1MCS2.
Source
Wild-type Citrobacter freundii ATCC 8090 was purchased from China Center of Industrial Culture Collection (CICC, China). For the construction of pBBR1MCS2-ppk1, genomic DNA of Citrobacter f.reundii was used as the template to PCR-amplify ppk1 with primers.
References
Wang X , Wang X , Hui K , et al. Highly Effective Polyphosphate Synthesis, Phosphate Removal and Concentration Using Engineered Environmental Bacteria Based on a Simple Solo Medium-copy Plasmid Strategy[J]. Environmental Science & Technology, 2017:acs.est.7b04532.