Difference between revisions of "Part:BBa K3221002:Design"

 
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===Design Notes===
 
===Design Notes===
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There are two domains in this parts, NK and HisG. They are relatively independent, so we can devide them by PCR.
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This part only have HisG domain in it. There is no tags and restriction sites in this part.
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===Source===
 
===Source===
  
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We got this part by gene synthesis. And it can also be found in the genome of Cordyceps militaris.
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We got this part from PCR, and it is a portion of CNS3. And it can also be found in the genome of Cordyceps militaris.
  
 
===References===
 
===References===
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Xia Y , Luo F , Shang Y , et al. Fungal Cordycepin Biosynthesis Is Coupled with the Production of the Safeguard Molecule Pentostatin[J]. Cell Chemical Biology, 2017:S2451945617303276.

Latest revision as of 09:20, 3 October 2019


HisG-only


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part only have HisG domain in it. There is no tags and restriction sites in this part.

Source

We got this part from PCR, and it is a portion of CNS3. And it can also be found in the genome of Cordyceps militaris.

References

Xia Y , Luo F , Shang Y , et al. Fungal Cordycepin Biosynthesis Is Coupled with the Production of the Safeguard Molecule Pentostatin[J]. Cell Chemical Biology, 2017:S2451945617303276.