Difference between revisions of "Part:BBa K2967015"

 
 
(One intermediate revision by the same user not shown)
Line 4: Line 4:
  
 
Firefly luciferase is luciferase from Photinus pyralis. By oxidation of substrate luciferin light is produced. Luciferase thus acts as a reporter protein when connected to other proteins or promoters.
 
Firefly luciferase is luciferase from Photinus pyralis. By oxidation of substrate luciferin light is produced. Luciferase thus acts as a reporter protein when connected to other proteins or promoters.
 +
 +
 +
'''Figure 3. The response to NO of Pyear-luc in E. coli Nissle 1917 .''' Histogram of Luminescence(RLU): pcdfduet-1 blank, Pyear-luc without SNP, pcdfduet-1 blank, Pyear-luc with 100μM SNP.
 +
 +
[1] Ljung T, Herulf M, Beijer E, et al. Rectal nitric oxide assessment in children with Crohn disease and ulcerative colitis. Indicator of ileocaecal and colorectal affection[J]. Scandinavian journal of gastroenterology, 2001, 36(10): 1073-1076.
 +
 +
Surprisingly, the luciferase in group without SNP was also be activated with undetermined mechanisms. We speculated that it may be caused by leakage expression of promoter PyeaR . Due to this reason, we also constructed an improvement of PyeaR based NO sensor which inserted an extra NOBS downstream of PyeaR to create a ‘roadblocking’ effect. The leakage is reduced by nineteen percent. Please click to “improvement biobricks<sub>”</sub> for more details.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 13:36, 2 October 2019


firefly luciferase

Firefly luciferase is luciferase from Photinus pyralis. By oxidation of substrate luciferin light is produced. Luciferase thus acts as a reporter protein when connected to other proteins or promoters.


Figure 3. The response to NO of Pyear-luc in E. coli Nissle 1917 . Histogram of Luminescence(RLU): pcdfduet-1 blank, Pyear-luc without SNP, pcdfduet-1 blank, Pyear-luc with 100μM SNP.

[1] Ljung T, Herulf M, Beijer E, et al. Rectal nitric oxide assessment in children with Crohn disease and ulcerative colitis. Indicator of ileocaecal and colorectal affection[J]. Scandinavian journal of gastroenterology, 2001, 36(10): 1073-1076.

Surprisingly, the luciferase in group without SNP was also be activated with undetermined mechanisms. We speculated that it may be caused by leakage expression of promoter PyeaR . Due to this reason, we also constructed an improvement of PyeaR based NO sensor which inserted an extra NOBS downstream of PyeaR to create a ‘roadblocking’ effect. The leakage is reduced by nineteen percent. Please click to “improvement biobricks” for more details.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]