Difference between revisions of "Part:BBa K2967015"
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Firefly luciferase is luciferase from Photinus pyralis. By oxidation of substrate luciferin light is produced. Luciferase thus acts as a reporter protein when connected to other proteins or promoters. | Firefly luciferase is luciferase from Photinus pyralis. By oxidation of substrate luciferin light is produced. Luciferase thus acts as a reporter protein when connected to other proteins or promoters. | ||
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| + | '''Figure 3. The response to NO of Pyear-luc in E. coli Nissle 1917 .''' Histogram of Luminescence(RLU): pcdfduet-1 blank, Pyear-luc without SNP, pcdfduet-1 blank, Pyear-luc with 100μM SNP. | ||
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| + | [1] Ljung T, Herulf M, Beijer E, et al. Rectal nitric oxide assessment in children with Crohn disease and ulcerative colitis. Indicator of ileocaecal and colorectal affection[J]. Scandinavian journal of gastroenterology, 2001, 36(10): 1073-1076. | ||
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| + | Surprisingly, the luciferase in group without SNP was also be activated with undetermined mechanisms. We speculated that it may be caused by leakage expression of promoter PyeaR . Due to this reason, we also constructed an improvement of PyeaR based NO sensor which inserted an extra NOBS downstream of PyeaR to create a ‘roadblocking’ effect. The leakage is reduced by nineteen percent. Please click to “improvement biobricks<sub>”</sub> for more details. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 13:35, 2 October 2019
firefly luciferase
Firefly luciferase is luciferase from Photinus pyralis. By oxidation of substrate luciferin light is produced. Luciferase thus acts as a reporter protein when connected to other proteins or promoters.
Figure 3. The response to NO of Pyear-luc in E. coli Nissle 1917 . Histogram of Luminescence(RLU): pcdfduet-1 blank, Pyear-luc without SNP, pcdfduet-1 blank, Pyear-luc with 100μM SNP.
[1] Ljung T, Herulf M, Beijer E, et al. Rectal nitric oxide assessment in children with Crohn disease and ulcerative colitis. Indicator of ileocaecal and colorectal affection[J]. Scandinavian journal of gastroenterology, 2001, 36(10): 1073-1076.
Surprisingly, the luciferase in group without SNP was also be activated with undetermined mechanisms. We speculated that it may be caused by leakage expression of promoter PyeaR . Due to this reason, we also constructed an improvement of PyeaR based NO sensor which inserted an extra NOBS downstream of PyeaR to create a ‘roadblocking’ effect. The leakage is reduced by nineteen percent. Please click to “improvement biobricks” for more details. Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]