Difference between revisions of "Part:BBa K3139010:Design"

 
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===Design Notes===
 
===Design Notes===
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Codon optimization was carried out for the expression in Serratia marcescens.
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Previous studies showed S. marcescens strain from which secB was deleted did not secrete HasA, SecB is required for HasA secretion in E.coli.  
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HasA is able to fold within the cyto-plasm and that it ceases to be secretion competent once folded.n
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HasA is able to fold within the cyto-plasm and that it ceases to be secretion competent once folded. Prevent HasA folding and favor the productive interaction of the C-terminal secretion signal with the ABC apparatus for efficient secretion.
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Prevent HasA folding and favor the productive interaction of the C-terminal secretion signal with the ABC apparatus for efficient secretion.
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===Source===
 
===Source===
  
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We found the coding sequence of HasA from literature, we modified this sequence and send it for stythesis.
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We found the coding sequence of HasA from NCBI, and send it for stythesis.
  
 
===References===
 
===References===

Latest revision as of 13:33, 2 October 2019


hasA hemophore coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Previous studies showed S. marcescens strain from which secB was deleted did not secrete HasA, SecB is required for HasA secretion in E.coli. HasA is able to fold within the cyto-plasm and that it ceases to be secretion competent once folded. Prevent HasA folding and favor the productive interaction of the C-terminal secretion signal with the ABC apparatus for efficient secretion.

Source

We found the coding sequence of HasA from NCBI, and send it for stythesis.

References