Difference between revisions of "Part:BBa K2992045"

 
Line 3: Line 3:
 
<partinfo>BBa_K2992045 short</partinfo>
 
<partinfo>BBa_K2992045 short</partinfo>
  
−
FAST reporter construct with P<i>thl</i> 5-UTR+RBS.
 
−
 
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
−
 
+
This parts entry represents a FAST reporter construct under the regulatory control of P<i>tthl</i> for the thiolase gene of <i>C. acetobutylicum</i>. The construct comprises the strong clostridial promoter P<i>thl</i> [https://parts.igem.org/Part:BBa_ K2715010BBa_ K2715010] coupled with its associated 5’-UTR [https://parts.igem.org/Part:BBa_ K2715019 BBa_ K2715019] and RBS [https://parts.igem.org/Part:BBa_K2992009 BBa_K2992009], driving the expression of the fluorescent reporter gene FAST [https://parts.igem.org/Part:BBa_K2992000 BBa_K2992000]. Transcriptional terminator occurs through the activity of the strong clostridial terminator T<i>Fad</i> [https://parts.igem.org/Part:BBa_K2284012 BBa_K2284012]. FAST is one of the few fluorescent reporters available for effective use in anaerobic organisms. FAST is derived from Halorhodospira halophila and has been codon optimised for fluorescence studies in the genus <i>Clostridium</i>. In our project we couple FAST with the natural promoters of the BotR regulon thus linking reporter fluorescence with botulinum neurotoxin production. In doing so, we hoped to generate our surrogate host strain as a model for predicting neurotoxin production in foodstuffs following food manufacturing processes.  <br><br>
 
<!-- -->
 
<!-- -->
 +
===Characterisation===
 +
Data incoming.
 +
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2992045 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2992045 SequenceAndFeatures</partinfo>
 +
 +
===References===
 +
Heap modular
 +
Street et al 2019. Update
  
  

Revision as of 12:41, 1 October 2019


FAST reporter construct with Pthl 5-UTR+RBS.

Usage and Biology

This parts entry represents a FAST reporter construct under the regulatory control of Ptthl for the thiolase gene of C. acetobutylicum. The construct comprises the strong clostridial promoter Pthl K2715010BBa_ K2715010 coupled with its associated 5’-UTR K2715019 BBa_ K2715019 and RBS BBa_K2992009, driving the expression of the fluorescent reporter gene FAST BBa_K2992000. Transcriptional terminator occurs through the activity of the strong clostridial terminator TFad BBa_K2284012. FAST is one of the few fluorescent reporters available for effective use in anaerobic organisms. FAST is derived from Halorhodospira halophila and has been codon optimised for fluorescence studies in the genus Clostridium. In our project we couple FAST with the natural promoters of the BotR regulon thus linking reporter fluorescence with botulinum neurotoxin production. In doing so, we hoped to generate our surrogate host strain as a model for predicting neurotoxin production in foodstuffs following food manufacturing processes.

Characterisation

Data incoming.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 330

References

Heap modular Street et al 2019. Update