Difference between revisions of "Part:BBa K2992042"

 
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<partinfo>BBa_K2992042 short</partinfo>
 
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Promoterless FAST construct.  
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This parts entry represents a promoterless FAST reporter construct. The construct comprises the fluorescent reporter gene FAST [], coupled with the strong clostridial terminator T<i>Fad</i>. FAST is one of the few fluorescent reporters available for effective use in anaerobic organisms. FAST is derived from Halorhodospira halophila and has been codon optimised for fluorescence studies in the genus Clostridium. In our project we couple FAST with the natural promoters of the BotR regulon thus linking reporter fluorescence with botulinum neurotoxin production. In doing so, we hoped to generate our surrogate host strain as a model for predicting neurotoxin production in foodstuffs following food manufacturing processes.  <br><br>
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===Usage and Biology===
 
===Usage and Biology===
  
 
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===Characterisation===
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Data incoming.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2992042 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2992042 SequenceAndFeatures</partinfo>
  
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===References===
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Street et al 2019. Update
  
 
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Revision as of 11:08, 1 October 2019


Promoterless FAST reporter construct

This parts entry represents a promoterless FAST reporter construct. The construct comprises the fluorescent reporter gene FAST [], coupled with the strong clostridial terminator TFad. FAST is one of the few fluorescent reporters available for effective use in anaerobic organisms. FAST is derived from Halorhodospira halophila and has been codon optimised for fluorescence studies in the genus Clostridium. In our project we couple FAST with the natural promoters of the BotR regulon thus linking reporter fluorescence with botulinum neurotoxin production. In doing so, we hoped to generate our surrogate host strain as a model for predicting neurotoxin production in foodstuffs following food manufacturing processes.

Usage and Biology

Characterisation

Data incoming.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 155

References

Street et al 2019. Update