Difference between revisions of "Part:BBa K2992041"
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<partinfo>BBa_K2992041 short</partinfo> | <partinfo>BBa_K2992041 short</partinfo> | ||
| − | 5-UTR containing RBS for ha33 gene from <i>C. botulinum</i>. | + | 5-UTR containing RBS for <i>ha33</i> gene from <i>C. botulinum</i>. |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 10:41, 1 October 2019
5-UTR containing RBS for ha33 gene from C. botulinum
5-UTR containing RBS for ha33 gene from C. botulinum.
Usage and Biology
This parts entry represents the 5’-UTR containing the RBS for the hemagglutinin component of the botulinum neurotoxin complex from C. botulinum. Since ha33 is natural constituent of the BotR regulon, we chose this promoter to control the expression of our FAST reporter construct thus linking reporter fluorescence with botulinum neurotoxin production. In doing so, we hoped to generate our surrogate host strain as a model for predicting neurotoxin production in foodstuffs following food manufacturing processes.
Characterisation
Data incoming.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Dupuy and Matamouros, 2006 Raffestin et al 2009 update