Difference between revisions of "Part:BBa K2967011"
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It is the sequence of inducible promoter hrpL. HrpR(BBa_K2967008) protein binds to HrpS(BBa_K2967009) protein forming a complex and then triggering the transcription of induced promoter hrpL. This functions like a AND logic gate to open an gene circuit. | It is the sequence of inducible promoter hrpL. HrpR(BBa_K2967008) protein binds to HrpS(BBa_K2967009) protein forming a complex and then triggering the transcription of induced promoter hrpL. This functions like a AND logic gate to open an gene circuit. | ||
| + | the hrpL promoter (PhrpL) contains a functional UAS between positions −147 to −36 relative to the transcription start-site, to which purified HrpR, HrpS and HrpRS bind in electrophoretic mobility shift assays. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 10:43, 30 September 2019
The inducible promoter, PhrpL
It is the sequence of inducible promoter hrpL. HrpR(BBa_K2967008) protein binds to HrpS(BBa_K2967009) protein forming a complex and then triggering the transcription of induced promoter hrpL. This functions like a AND logic gate to open an gene circuit. the hrpL promoter (PhrpL) contains a functional UAS between positions −147 to −36 relative to the transcription start-site, to which purified HrpR, HrpS and HrpRS bind in electrophoretic mobility shift assays.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]