Difference between revisions of "Part:BBa K3128032"
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<partinfo>BBa_K3128032 short</partinfo> | <partinfo>BBa_K3128032 short</partinfo> | ||
| − | Clickable OmpX is an outer membrane protein whose C and N | + | Clickable OmpX is an outer membrane protein whose C and N terminal in the intracellular domain. It can be used as a scaffold if an unnatural amino acid is to be introduced. |
This can be done through the presence of the amber stop codon TAG in the third loop OmpX. With a specific tRNA, an amino acid functionalized with an azide will be integrated, which can be used for the SPAAC click chemistry reaction with DBCO / DIBO functional groups. Once expressed, this biobrick will serve as a positive control to check the click for biobrick BBa_K3128015 et BBa_3128016 during the expression test | This can be done through the presence of the amber stop codon TAG in the third loop OmpX. With a specific tRNA, an amino acid functionalized with an azide will be integrated, which can be used for the SPAAC click chemistry reaction with DBCO / DIBO functional groups. Once expressed, this biobrick will serve as a positive control to check the click for biobrick BBa_K3128015 et BBa_3128016 during the expression test | ||
Revision as of 17:06, 29 September 2019
COMP : Clickable OmpX
Clickable OmpX is an outer membrane protein whose C and N terminal in the intracellular domain. It can be used as a scaffold if an unnatural amino acid is to be introduced. This can be done through the presence of the amber stop codon TAG in the third loop OmpX. With a specific tRNA, an amino acid functionalized with an azide will be integrated, which can be used for the SPAAC click chemistry reaction with DBCO / DIBO functional groups. Once expressed, this biobrick will serve as a positive control to check the click for biobrick BBa_K3128015 et BBa_3128016 during the expression test
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]