Difference between revisions of "Part:BBa K2973016:Design"

 
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iGEM Part Registry
 
iGEM Part Registry
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NCBI Mycobacterium tuberculosis Genome, Strain H37Rv, Accession Number: NC_000962
  
 
===References===
 
===References===
 +
https://www.ncbi.nlm.nih.gov/tools/primer-blast/

Latest revision as of 19:54, 28 September 2019


IS6110 Forward Primer with 5' Overhang


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The primer design was accomplished using the Primer3 software (Primer-BLAST). The T7 Promoter on the 5' end of the sequence allows for downstream transcription of the amplified product by a T7 RNA Polymerase.


Source

iGEM Part Registry NCBI Mycobacterium tuberculosis Genome, Strain H37Rv, Accession Number: NC_000962

References

https://www.ncbi.nlm.nih.gov/tools/primer-blast/