Difference between revisions of "Part:BBa K2938003"

 
 
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===Description===
  
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Pesticidal crystal protein Cyt1Aa is a 27 kDa protein which can be isolated from Bacillus thuringiensis subsp. israelensis (Bti).  
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Pesticidal crystal protein Cyt1Aa is a 27 kDa protein which can be isolated from Bacillus thuringiensis subsp. israelensis (Bti).
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The pBtoxis is a toxin-encoding plasmid that its origin is the Bti and it encodes to four Cry toxins (Cry4Aa, Cry4Ba, Cry10Aa and Cry11Aa) and three Cyt toxins (Cyt1Aa, Cyt2Ba and Cyt1Ca)[1], which are specifically toxic against Aedes, Anopheles and Culex mosquitoes which are vectors to many diseases caused by parasites and viruses, such as Malaria, Zika and Dengue.
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Each of the toxins hold a low level of larvicidal effect but their combination has a synergetic effect which is stronger than the linear combination we expect. The Cyt1Aa toxin effect in the synergetic effect is mainly the prevention of resistance in the mosquito to the Cry toxins.
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The bigger Cry toxins, Cry4Aa and Cry4Ba have a conserved C-terminal end which helps the toxin fold correctly and form crystal structures.
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[[File:ToxinTable.png]]
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===Amplification===
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[[File:Cyt1Aa PCR.jpeg|200px|thumb|left|PCR  amplification of Cyt1Aa by PCR.]]
  
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===Usage and Biology===
 
  
 
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Latest revision as of 10:28, 28 September 2019


Cyt1Aa

Description

Pesticidal crystal protein Cyt1Aa is a 27 kDa protein which can be isolated from Bacillus thuringiensis subsp. israelensis (Bti). The pBtoxis is a toxin-encoding plasmid that its origin is the Bti and it encodes to four Cry toxins (Cry4Aa, Cry4Ba, Cry10Aa and Cry11Aa) and three Cyt toxins (Cyt1Aa, Cyt2Ba and Cyt1Ca)[1], which are specifically toxic against Aedes, Anopheles and Culex mosquitoes which are vectors to many diseases caused by parasites and viruses, such as Malaria, Zika and Dengue. Each of the toxins hold a low level of larvicidal effect but their combination has a synergetic effect which is stronger than the linear combination we expect. The Cyt1Aa toxin effect in the synergetic effect is mainly the prevention of resistance in the mosquito to the Cry toxins. The bigger Cry toxins, Cry4Aa and Cry4Ba have a conserved C-terminal end which helps the toxin fold correctly and form crystal structures.

ToxinTable.png


Amplification

PCR amplification of Cyt1Aa by PCR.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 419
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 419
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 717
    Illegal BamHI site found at 96
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 419
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 419
  • 1000
    COMPATIBLE WITH RFC[1000]