Difference between revisions of "Part:BBa K2992036:Design"
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Amplified unmodified from the genome sequences of the host organisms. The <i>ctfA</i> and <i>ctfB</i> genes from the iGEM 2015 entries [https://parts.igem.org/Part:BBa_ M36581 BBa_ M36581] and [https://parts.igem.org/Part:BBa_ M36582 BBa_ M36582] list the reverse complements of the coding sequences. In our entry we use the same sequences but in the correct 5’-3’ orientation. The RBS for <i>ctfB</i> is contained within the coding sequence for <i>ctfA</i>. | Amplified unmodified from the genome sequences of the host organisms. The <i>ctfA</i> and <i>ctfB</i> genes from the iGEM 2015 entries [https://parts.igem.org/Part:BBa_ M36581 BBa_ M36581] and [https://parts.igem.org/Part:BBa_ M36582 BBa_ M36582] list the reverse complements of the coding sequences. In our entry we use the same sequences but in the correct 5’-3’ orientation. The RBS for <i>ctfB</i> is contained within the coding sequence for <i>ctfA</i>. | ||
Latest revision as of 12:13, 26 September 2019
Design considerations
Amplified unmodified from the genome sequences of the host organisms. The ctfA and ctfB genes from the iGEM 2015 entries M36581 BBa_ M36581 and M36582 BBa_ M36582 list the reverse complements of the coding sequences. In our entry we use the same sequences but in the correct 5’-3’ orientation. The RBS for ctfB is contained within the coding sequence for ctfA.