Difference between revisions of "Part:BBa K2927066"

Revision as of 14:41, 25 September 2019

T7 promoter-guide RNA-crRNA 6-mutated crRNA 3

The sequence we designed is used for generating crRNA 6 and mutated crRNA 3. CrRNA 6 is used for African swine fever (ASFV) detection. Furthermore, mutated crRNA 3 is the sequence derived from crRNA 3 for negative control to test whether our homemade Cas12a protein works functionally and normally. Kpn1 is the cutting site that helps us to separate crRNA 6 and mutated crRNA 3.

The sequence we designed is used for generating crRNA 6 and mutated crRNA 3. CrRNA 6 is used for African swine fever virus (ASFV) detection. Furthermore, mutated crRNA 3 is the sequence derived from crRNA 3 for negative control to test whether our purified Cas12a protein works functionally and normally. Kpn1 is the cutting site that helps us to separate crRNA 6 and mutated crRNA 3.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 The sequence we designed is used for generating crRNA 6 and mutated crRNA 3. CrRNA 6 is used for African swine fever (ASFV) detection. Furthermore, mutated crRNA 3 is the sequence derived from crRNA 3 for negative control to test whether our homemade Cas12a protein works functionally and normally. Kpn1 is the cutting site that helps us to separate crRNA 6 and mutated crRNA 3.
+The sequence we designed is used for generating crRNA 6 and mutated crRNA 3. CrRNA 6 is used for African swine fever virus (ASFV) detection. Furthermore, mutated crRNA 3 is the sequence derived from crRNA 3 for negative control to test whether our purified Cas12a protein works functionally and normally. Kpn1 is the cutting site that helps us to separate crRNA 6 and mutated crRNA 3.
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