Difference between revisions of "Part:BBa K2927064"

 
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The sequence we designed is used for generating crRNA 4 and mutated crRNA 5. CrRNA 4 is used for African swine fever (ASFV) detection. Furthermore, mutated crRNA 5 is the sequence derived from crRNA 5 for negative control to test whether our homemade Cas12a protein works functionally and normally. Kpn1 is the cutting site that helps us to separate crRNA 4 and mutated crRNA 5.
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The sequence we designed is used for generating crRNA 4 and mutated crRNA 5. CrRNA 4 is used for African swine fever virus (ASFV) detection. Furthermore, mutated crRNA 5 is the sequence derived from crRNA 5 for negative control to test whether our purified Cas12a protein works functionally and normally. Kpn1 is the cutting site that helps us to separate crRNA 4 and mutated crRNA 5.
  
 
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Revision as of 14:39, 25 September 2019


T7 promoter-guide RNA-crRNA 4-mutated crRNA 5

The sequence we designed is used for generating crRNA 4 and mutated crRNA 5. CrRNA 4 is used for African swine fever virus (ASFV) detection. Furthermore, mutated crRNA 5 is the sequence derived from crRNA 5 for negative control to test whether our purified Cas12a protein works functionally and normally. Kpn1 is the cutting site that helps us to separate crRNA 4 and mutated crRNA 5.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]