Difference between revisions of "Part:BBa K2927022"

Revision as of 14:34, 25 September 2019

Negative control sequence for crRNA 3 target

We have made a change on 5 nucleotides of crRNA 3 from 5’ end to make the mutated crRNA 3. It is the sequence for testing whether our purified Cas12a protein functions properly. We used it for a negative control of our project. Therefore, the sequence of mutated crRNA 3 is too short to be the functional protein. Thus, there is no risk for the spread out of ASFV from our project. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2927022 short</partinfo>
-We changed 5 nucleotides of crRNA 1 from 5’ end to make the mutated crRNA 3. It is the sequence for testing whether homemade Cas12a protein functions properly. We used it for a negative control of our project. Therefore, the sequence of mutated crRNA 3 is too short to become the functional protein. There is no concentration for spread out ASFV of our project.
+We have made a change on 5 nucleotides of crRNA 3 from 5’ end to make the mutated crRNA 3. It is the sequence for testing whether our purified Cas12a protein functions properly. We used it for a negative control of our project. Therefore, the sequence of mutated crRNA 3 is too short to be the functional protein. Thus, there is no risk for the spread out of ASFV from our project.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===