Difference between revisions of "Part:BBa K2916007"
Line 3: Line 3:
 
<partinfo>BBa_K2916007 short</partinfo>
 
<partinfo>BBa_K2916007 short</partinfo>
  
−
Glycyl-tRNA synthetase is a fusion protein that is used in the PURE and OnePot PURE cell-free systems.This protein includes both GlyRS-alpha and GlyRS-beta domains.
 
  
−
<!-- Add more about the biology of this part here
+
Phenylalanyl-tRNA synthetase is a protein that is used in the PURE and OnePot PURE cell-free systems.
 +
 
 +
<!-- Add more about the biology of this part here-->
 
===Usage and Biology===
 
===Usage and Biology===
 +
 +
Used in OnePot PURE
 +
  
 
<!-- -->
 
<!-- -->

Revision as of 10:46, 24 September 2019


PheRS protein equipped with a 6x HIS affinity tag


Phenylalanyl-tRNA synthetase is a protein that is used in the PURE and OnePot PURE cell-free systems.

Usage and Biology

Used in OnePot PURE


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2424
    Illegal BamHI site found at 31
    Illegal BamHI site found at 1772
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1162
    Illegal AgeI site found at 1155
    Illegal AgeI site found at 2077
    Illegal AgeI site found at 2929
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2875
    Illegal SapI.rc site found at 198
    Illegal SapI.rc site found at 2851