Difference between revisions of "Part:BBa K2933010"
 
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<partinfo>BBa_K2933010 short</partinfo>
 
<partinfo>BBa_K2933010 short</partinfo>
  
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This part encodes a protein called MUS-2, which is a metallo-beta-lactamase of subclass B2.
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This part encodes a protein called MUS-2, which is a metallo-beta-lactamase of subclass B1.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
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===Usage and Biology===
 
===Usage and Biology===
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MUS-2 is 啊
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MUS-2 is a novel variant of the chromosome-encoded β-lactamase MUS-1 in M. odoratimimus, which has been isolated from livestock animals in Lebanon. It could be sure that animals could be a reservoir for such genes and are a source of new and/or emerging multidrug-resistant bacteria and due to possible links with animals, humans may have high risk factors for colonization/infection with such bacteria. Bacteria that carry the enzyme are potentially harmful to humans.
  
 
===Molecular cloning===
 
===Molecular cloning===
 
We used the vector pGEX-6p-1 to construct our expression plasmid.  
 
We used the vector pGEX-6p-1 to construct our expression plasmid.  
 
<p style="text-align: center;">
 
<p style="text-align: center;">
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   [[File:TJUSLS China--MUS-2-PCR.png.png|500px]]<br>
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   [[File:TJUSLS China--MUS-2-PCR.png|500px]]<br>
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'''Figure 1.'''  Left: The PCR result of MUS-2. Right: The verification results by enzyme digestion.<br>
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</p>
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After verification, it was determined that the construction is successful.
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===References===
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[1]Al-Bayssari C, Gupta SK, Dabboussi F, Hamze M, Rolain JM. MUS-2, a novel variant of the chromosome-encoded β-lactamase MUS-1, from Myroides odoratimimus. New Microbes New Infect. 2015 Jun 27;7:67-71.
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[2] Mammeri H, Bellais S, Nordmann P. Chromosome-encoded beta-lactamases TUS-1 and MUS-1 from Myroides odoratus and Myroides odoratimimus (formerly Flavobacterium odoratum), new members of the lineage of molecular subclass B1 metalloenzymes. Antimicrob Agents Chemother. 2002 Nov;46(11):3561-7.

Latest revision as of 07:19, 24 September 2019


subclass B1 metallo-beta-lactamase MUS-2, codon optimized in E. coli

This part encodes a protein called MUS-2, which is a metallo-beta-lactamase of subclass B1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

MUS-2 is a novel variant of the chromosome-encoded β-lactamase MUS-1 in M. odoratimimus, which has been isolated from livestock animals in Lebanon. It could be sure that animals could be a reservoir for such genes and are a source of new and/or emerging multidrug-resistant bacteria and due to possible links with animals, humans may have high risk factors for colonization/infection with such bacteria. Bacteria that carry the enzyme are potentially harmful to humans.

Molecular cloning

We used the vector pGEX-6p-1 to construct our expression plasmid.

TJUSLS China--MUS-2-PCR.png
Figure 1. Left: The PCR result of MUS-2. Right: The verification results by enzyme digestion.

After verification, it was determined that the construction is successful.

References

[1]Al-Bayssari C, Gupta SK, Dabboussi F, Hamze M, Rolain JM. MUS-2, a novel variant of the chromosome-encoded β-lactamase MUS-1, from Myroides odoratimimus. New Microbes New Infect. 2015 Jun 27;7:67-71.

[2] Mammeri H, Bellais S, Nordmann P. Chromosome-encoded beta-lactamases TUS-1 and MUS-1 from Myroides odoratus and Myroides odoratimimus (formerly Flavobacterium odoratum), new members of the lineage of molecular subclass B1 metalloenzymes. Antimicrob Agents Chemother. 2002 Nov;46(11):3561-7.