Difference between revisions of "Part:BBa K2927005"

Revision as of 16:08, 23 September 2019

LbCas12a

CRISPR Cas12a system is one of the bacterial adaptive immune systems. Cas12a protein is the RNA-guided enzyme that binds and cut DNA. When Cas12a protein bind with the specific crRNA, it will be activated. After Cas12a protein is activated, it will cut the target DNA as well as non-specific single-strand DNA (ssDNA), this certain function is used in our project. We designed six crRNA which is derived from the African swine fever virus (ASFV) to detect the DNA of the virus. This sequence is just a part of DNA sequence of the vp72 membrane protein of ASFV.

Reference
Janice S. Chen, Enbo Ma, Lucas B. Harrington, Maria Da Costa, Xinran Tian, Joel M. Palefsky, Jennifer A. Doudna, Chen et al., Science 360, 436–439 (2018) Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1371
Illegal BglII site found at 2108
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 3022
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1435

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2927005 short</partinfo>
-CRISPR Cas12a system is one of the bacterial adaptive immune systems. Cas12a proteins are RNA-guided enzymes that bind and cut DNA. When Cas12a protein bind with the specific crRNA, it is activated. After Cas12a protein is activated, it will cut the target DNA as well as non-specific single-strand DNA (ssDNA). We used the function of our project. We designed six crRNA which is derived from the African swine fever virus (ASFV) to detect the DNA of the virus.
+CRISPR Cas12a system is one of the bacterial adaptive immune systems. Cas12a protein is the RNA-guided enzyme that binds and cut DNA. When Cas12a protein bind with the specific crRNA, it will be activated. After Cas12a protein is activated, it will cut the target DNA as well as non-specific single-strand DNA (ssDNA), this certain function is used in our project. We designed six crRNA which is derived from the African swine fever virus (ASFV) to detect the DNA of the virus. This sequence is just a part of DNA sequence of the vp72 membrane protein of ASFV.
 '''Reference'''<br>