Difference between revisions of "Part:BBa K2927002"
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<partinfo>BBa_K2927002 short</partinfo>
 
<partinfo>BBa_K2927002 short</partinfo>
  
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10 times histidine is made up of 10 histidines connected. Because of the affinity with Ni, it makes composition (10X His-MBP-TEV-Cas12a) bind on the Ni column. It’s used for Cas12a protein purification. The 10X histidine is used by E.coli.
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10 times histidine is made up of 10 connected histidines. Because of the affinity with Ni, it makes the composition (10X His-MBP-TEV-Cas12a) bind on the Ni column. It’s used for Cas12a protein purification.  
  
 
'''Reference'''<br>
 
'''Reference'''<br>

Revision as of 15:43, 23 September 2019

10X His

10 times histidine is made up of 10 connected histidines. Because of the affinity with Ni, it makes the composition (10X His-MBP-TEV-Cas12a) bind on the Ni column. It’s used for Cas12a protein purification.

Reference
Janice S. Chen, Enbo Ma, Lucas B. Harrington, Maria Da Costa, Xinran Tian, Joel M. Palefsky, Jennifer A. Doudna, Chen et al., Science 360, 436–439 (2018) Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]