Difference between revisions of "Part:BBa K3195003:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | Because Cathespin B gene is in eukaryotes,we optimized its condons for better prokaryotic expression.Considering the following seperation and purification,we add | + | Because Cathespin B gene is in eukaryotes,we optimized its condons for better prokaryotic expression.Considering the following seperation and purification,we add 6X His at its tail.In addition,we cut off the signal peptide. |
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===Source=== | ===Source=== |
Revision as of 15:18, 23 September 2019
His-tagged Cathepsin B
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 369
Illegal XhoI site found at 958 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 463
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Because Cathespin B gene is in eukaryotes,we optimized its condons for better prokaryotic expression.Considering the following seperation and purification,we add 6X His at its tail.In addition,we cut off the signal peptide.
Source
Cathespin B gene from Branchiostoma belcheri tsingtauense