Difference between revisions of "Part:BBa K2933020"

 
(→‎References)
 
(5 intermediate revisions by 2 users not shown)
Line 6: Line 6:
  
  
−
<!-- Add more about the biology of this part here
 
−
===Usage and Biology===
 
  
 
<!-- -->
 
<!-- -->
Line 15: Line 13:
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
−
===Functional Parameters===
+
`2===Functional Parameters===
 
<partinfo>BBa_K2933020 parameters</partinfo>
 
<partinfo>BBa_K2933020 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
===Usage and Biology===
 +
Bacteria with IMP-type enzymes have spread through out the world, and the IMP group now has more than 50 variants
 +
These enzymes possess a broad substrate specificity and a high affinity for cephalosporins and carbapenems but a low activity toward temocillin<br>
 +
===References===
 +
[1]Purification, crystallization and preliminary X-ray analysis of IMP-18, a class B carbapenemase from Pseudomonas aeruginosa.Furuyama T, Ishii Y, Ohya N, Tateda K, Hanson ND, Shimizu-Ibuka A.Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Dec;69(Pt 12):1397-400.
 +
 +
===Molecular cloning===
 +
First, we used the vector pGEX-6p-1 to construct our expression plasmid. And then we converted the plasmid constructed to ''E. coli'' DH5α to expand the plasmid largely.<br>
 +
<p style="text-align: center;">
 +
  [[File:IMP-71-PCR.png|400px]]<br>
 +
'''Figure 1.'''  Left: The PCR result of IMP-71. Right: The verification results by enzyme digestion.<br>
 +
</p>
 +
After verification, it was determined that the construction is successful. We converted the plasmid to ''E. coli'' BL21(DE3) for expression and purification.<br>

Latest revision as of 14:56, 23 September 2019


subclass B1 metallo-beta-lactamase IMP-71, codon optimized in E. coli

This part encodes a protein called IMP-71, which is a metallo-beta-lactamase of subclass B1.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 724
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 724
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 724
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 724
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

Bacteria with IMP-type enzymes have spread through out the world, and the IMP group now has more than 50 variants These enzymes possess a broad substrate specificity and a high affinity for cephalosporins and carbapenems but a low activity toward temocillin

References

[1]Purification, crystallization and preliminary X-ray analysis of IMP-18, a class B carbapenemase from Pseudomonas aeruginosa.Furuyama T, Ishii Y, Ohya N, Tateda K, Hanson ND, Shimizu-Ibuka A.Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Dec;69(Pt 12):1397-400.

Molecular cloning

First, we used the vector pGEX-6p-1 to construct our expression plasmid. And then we converted the plasmid constructed to E. coli DH5α to expand the plasmid largely.

IMP-71-PCR.png
Figure 1. Left: The PCR result of IMP-71. Right: The verification results by enzyme digestion.

After verification, it was determined that the construction is successful. We converted the plasmid to E. coli BL21(DE3) for expression and purification.