Difference between revisions of "Part:BBa K2933008"
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===Usage and Biology=== | ===Usage and Biology=== | ||
blaPEDO-1, a new carbapenem-hydrolyzing beta-lactamases, this enzyme has not yet emerged in clinical settings but constitute potential carbap- enem resistance determinants in pathogenic bacterial species, as demonstrated by their ability to confer resistance to ampi- cillin and various cephalosporins, as well as reduced suscepti- bility to carbapenems, once expressed in E. coli. | blaPEDO-1, a new carbapenem-hydrolyzing beta-lactamases, this enzyme has not yet emerged in clinical settings but constitute potential carbap- enem resistance determinants in pathogenic bacterial species, as demonstrated by their ability to confer resistance to ampi- cillin and various cephalosporins, as well as reduced suscepti- bility to carbapenems, once expressed in E. coli. | ||
| − | + | ===References=== | |
| + | [1]Dereje Dadi Gudeta, Valeria Bortolaia,The Soil Microbiota Harbors a Diversity of Carbapenem-Hydrolyzing β-Lactamases of Potential Clinical Relevance[J]，Antimicrobial Agents and chemotherapy,January 2016<br> | ||
===Molecular cloning=== | ===Molecular cloning=== | ||
First, we used the vector pGEX-6p-1 to construct our expression plasmid. And then we converted the plasmid constructed to ''E. coli'' DH5α to expand the plasmid largely.<br> | First, we used the vector pGEX-6p-1 to construct our expression plasmid. And then we converted the plasmid constructed to ''E. coli'' DH5α to expand the plasmid largely.<br> | ||
Latest revision as of 12:07, 23 September 2019
subclass B1 metallo-beta-lactamase PEDO-1, codon optimized in E. coli
This part encodes a protein called PEDO-1, which is a metallo-beta-lactamase of subclass B1.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 537
Illegal PstI site found at 823 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 537
Illegal PstI site found at 823 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 537
Illegal PstI site found at 823 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 537
Illegal PstI site found at 823 - 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
blaPEDO-1, a new carbapenem-hydrolyzing beta-lactamases, this enzyme has not yet emerged in clinical settings but constitute potential carbap- enem resistance determinants in pathogenic bacterial species, as demonstrated by their ability to confer resistance to ampi- cillin and various cephalosporins, as well as reduced suscepti- bility to carbapenems, once expressed in E. coli.
References
[1]Dereje Dadi Gudeta, Valeria Bortolaia,The Soil Microbiota Harbors a Diversity of Carbapenem-Hydrolyzing β-Lactamases of Potential Clinical Relevance[J]，Antimicrobial Agents and chemotherapy,January 2016
Molecular cloning
First, we used the vector pGEX-6p-1 to construct our expression plasmid. And then we converted the plasmid constructed to E. coli DH5α to expand the plasmid largely.
Figure 1. Left: The PCR result of PEDO-1. Right: The verification results by enzyme digestion.