Difference between revisions of "Part:BBa K2926005"

 
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__NOTOC__
 
__NOTOC__
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{|width='80%'
 
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|-
 
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|width=35% valign='top'|
 
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[[Image:Terminator.png]]
 
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<partinfo>BBa_K2926005 parameters</partinfo>
 
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| width=50% valign='top' style='border: 1px solid black'|
 
 
<partinfo>BBa_K2926005 short</partinfo>
 
<partinfo>BBa_K2926005 short</partinfo>
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* long description
 
  
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|}
 
  
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'''Secondary Structure'''
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==Usage and Biology==
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<html>
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<p>
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TPs1 is the natural terminator of the tps1 gen in Saccharomyces cerevisiae,
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which is encoding the trehalose-6-phosphate synthetase.
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</p>
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2926005 SequenceAndFeatures</partinfo>
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__TOC__
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K2926003 parameters</partinfo>
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<!-- -->
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==Plasmid Design==
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<html>
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For the analysis and characterization of the GALL Promotor <a href="XXX">XXXX</a>
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in combiantion with the TPs1 Terminaror <a href="XXX">XXXX</a>,
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mCherry <a href="XXX">XXXX</a> was cloned beteen them using Gibson Assembly.
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The GALL Promotor and TPs1 Terminator where optained as a gBlock from IDT.
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  </html>
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===Sequencing Results===
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<html>
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The biobrick was analysed with Sanger Sequenceing to confirm its correct base sequence.
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</html>
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==Characterisation==
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<html>
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We followed the standard <a href="https://www.protocols.io/view/calibration-protocol-plate-reader-fluorescence-cal-6zrhf56">iGEM protocoll for GFP fluorescence calibration</a>,
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measuring our cultures against Flourescin.
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We performed the analysis with <i>E. coli DH5α</i> as well as <i>S. cerevisiae</i> cultures.
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<br>
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See <a href="XXX">BBa_K2926073</a> for the characterization results.
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</html>
  
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[[Image:Mfold-K2926005-1.png]]
 
  
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<hr>
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==References==
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'''Measurement'''
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* [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]
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Revision as of 21:21, 22 September 2019


TPs1 yeast terminator


Usage and Biology

TPs1 is the natural terminator of the tps1 gen in Saccharomyces cerevisiae, which is encoding the trehalose-6-phosphate synthetase.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Plasmid Design

For the analysis and characterization of the GALL Promotor XXXX in combiantion with the TPs1 Terminaror XXXX, mCherry XXXX was cloned beteen them using Gibson Assembly. The GALL Promotor and TPs1 Terminator where optained as a gBlock from IDT.


Sequencing Results

The biobrick was analysed with Sanger Sequenceing to confirm its correct base sequence.

Characterisation

We followed the standard iGEM protocoll for GFP fluorescence calibration, measuring our cultures against Flourescin. We performed the analysis with E. coli DH5α as well as S. cerevisiae cultures.
See BBa_K2926073 for the characterization results.


References