Difference between revisions of "Part:BBa K2933189"

 
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<partinfo>BBa_K2933189 parameters</partinfo>
 
<partinfo>BBa_K2933189 parameters</partinfo>
 
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===Usage and Biology===
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This composite part is made up with nine basic parts, T7 promoter, the RBS b， the linker h， His tag，the linker a, Sumo tag, linker b, the gene of CPS-1 and T7 terminator.It encodes a protein which is CPS-1 fused with His and Sumo tag. The fusion protein is about 45.2 kD. In order to gain the highly purified target protein, we add GST tag in N-terminal of CPS-1 and combine Sumo tag to increased protein solubility. The fusion protein can be cut off at the cutting site by Prescission Protease. It is convenient for us to purify our target protein.<br>

Revision as of 13:11, 21 September 2019


T7 promoter+RBS b+linker h+His+Linker a+Sumo+Linker b+CPS-1+T7 terminator

The part consists of T7 promoter,RBS and protein coding(His+Linker a+Sumo+Linker b+CPS-1)and the biological module can be built into E.coil for protein expression.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 390
    Illegal XbaI site found at 47
    Illegal PstI site found at 1026
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 390
    Illegal NheI site found at 167
    Illegal NheI site found at 1370
    Illegal PstI site found at 1026
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 390
    Illegal BglII site found at 279
    Illegal BglII site found at 1308
    Illegal BamHI site found at 478
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 390
    Illegal XbaI site found at 47
    Illegal PstI site found at 1026
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 390
    Illegal XbaI site found at 47
    Illegal PstI site found at 1026
    Illegal AgeI site found at 927
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

This composite part is made up with nine basic parts, T7 promoter, the RBS b， the linker h， His tag，the linker a, Sumo tag, linker b, the gene of CPS-1 and T7 terminator.It encodes a protein which is CPS-1 fused with His and Sumo tag. The fusion protein is about 45.2 kD. In order to gain the highly purified target protein, we add GST tag in N-terminal of CPS-1 and combine Sumo tag to increased protein solubility. The fusion protein can be cut off at the cutting site by Prescission Protease. It is convenient for us to purify our target protein.