Difference between revisions of "Part:BBa K2933177"
Line 18: | Line 18: | ||
<partinfo>BBa_K2933177 parameters</partinfo> | <partinfo>BBa_K2933177 parameters</partinfo> | ||
<!-- --> | <!-- --> | ||
+ | ===Usage and Biology=== | ||
+ | <br> | ||
+ | ===Molecular cloning=== | ||
+ | First, we used the vector pGEX-6p-1 and the vector to construct our expression plasmid. And then we converted the plasmid constructed to ''E. coli'' DH5α to expand the plasmid largely.<br> | ||
+ | <p style="text-align: center;"> | ||
+ | [[File:ARL-1-PCR.png|500px]]<br> | ||
+ | '''Figure 1.''' Left: The PCR result of ARL-1. Right: The verification results by enzyme digestion.<br> | ||
+ | </p> |
Revision as of 12:50, 21 September 2019
Tac promoter+RBS a+Linker g+GST+Linker e+ARL-1
This part consists of Tac promoter,RBS and protein coding sequence (GST+Linker e+ARL-1),and the biological module can be built into E.coli for protein expression.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1221
Illegal PstI site found at 1335
Illegal PstI site found at 1386 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1221
Illegal PstI site found at 1335
Illegal PstI site found at 1386 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1221
Illegal PstI site found at 1335
Illegal PstI site found at 1386 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1221
Illegal PstI site found at 1335
Illegal PstI site found at 1386 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 181
Usage and Biology
Molecular cloning
First, we used the vector pGEX-6p-1 and the vector to construct our expression plasmid. And then we converted the plasmid constructed to E. coli DH5α to expand the plasmid largely.
Figure 1. Left: The PCR result of ARL-1. Right: The verification results by enzyme digestion.