Difference between revisions of "Part:BBa K2933176"
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<partinfo>BBa_K2933176 parameters</partinfo> | <partinfo>BBa_K2933176 parameters</partinfo> | ||
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+ | ===Usage and Biology=== | ||
+ | <br> | ||
+ | ===Molecular cloning=== | ||
+ | First, we used the vector pGEX-6p-1 to construct our expression plasmid. And then we converted the plasmid constructed to ''E. coli'' DH5α to expand the plasmid largely.<br> | ||
+ | <p style="text-align: center;"> | ||
+ | [[File:PST-1-PCR.png]]<br> | ||
+ | '''Figure 1.''' a: The PCR result of PST-1. b: The verification results by enzyme digestion.<br> | ||
+ | </p> |
Revision as of 12:49, 21 September 2019
Tac promoter+RBS a+Linker g+GST+Linker e+PST-1
This part consists of Tac promoter,RBS and protein coding sequence (GST+Linker e+PST-1),and the biological module can be built into E.coli for protein expression.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 181
Usage and Biology
Molecular cloning
First, we used the vector pGEX-6p-1 to construct our expression plasmid. And then we converted the plasmid constructed to E. coli DH5α to expand the plasmid largely.
Figure 1. a: The PCR result of PST-1. b: The verification results by enzyme digestion.