Difference between revisions of "Part:BBa K2933175"
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<partinfo>BBa_K2933175 parameters</partinfo> | <partinfo>BBa_K2933175 parameters</partinfo> | ||
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+ | ===Usage and Biology=== | ||
+ | <br> | ||
+ | ===Molecular cloning=== | ||
+ | We insert VIM-66 gene into the standard vector then transfer it into E.coli. | ||
+ | [[File:VIM-66-PCR.jpeg|600px|center|]] | ||
+ | <p style="text-align: center;"> | ||
+ | '''Figure 1.''' Left: The result of PCR, Right:The result of double enzyme digestion verification.LaneM,Marker, Lane1, the plasmid with VIM-66, Lane2, after double enzyme verification |
Revision as of 12:47, 21 September 2019
Tac promoter+RBS a+Linker g+GST+Linker e+VIM-66
This part consists of Tac promoter,RBS and protein coding sequence (GST+Linker e+VIM-66),and the biological module can be built into E.coli for protein expression.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1532
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 181
Usage and Biology
Molecular cloning
We insert VIM-66 gene into the standard vector then transfer it into E.coli.
Figure 1. Left: The result of PCR, Right:The result of double enzyme digestion verification.LaneM,Marker, Lane1, the plasmid with VIM-66, Lane2, after double enzyme verification