Difference between revisions of "Part:BBa K2933175"

 
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<partinfo>BBa_K2933175 parameters</partinfo>
 
<partinfo>BBa_K2933175 parameters</partinfo>
 
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===Usage and Biology===
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<br>
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===Molecular cloning===
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We insert VIM-66 gene into the standard vector then transfer it into E.coli.
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  [[File:VIM-66-PCR.jpeg|600px|center|]] 
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<p style="text-align: center;">
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'''Figure 1.''' Left: The result of PCR, Right:The result of double enzyme digestion verification.LaneM,Marker, Lane1, the plasmid with VIM-66, Lane2, after double enzyme verification

Revision as of 12:47, 21 September 2019


Tac promoter+RBS a+Linker g+GST+Linker e+VIM-66

This part consists of Tac promoter,RBS and protein coding sequence (GST+Linker e+VIM-66),and the biological module can be built into E.coli for protein expression.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1532
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 181


Usage and Biology


Molecular cloning

We insert VIM-66 gene into the standard vector then transfer it into E.coli.

VIM-66-PCR.jpeg

Figure 1. Left: The result of PCR, Right:The result of double enzyme digestion verification.LaneM,Marker, Lane1, the plasmid with VIM-66, Lane2, after double enzyme verification