Difference between revisions of "Part:BBa K2916003:Design"
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Codon optimization : 1509 from A to G | Codon optimization : 1509 from A to G | ||
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| + | A second stop codon was added in compliance with iGEM standards. | ||
===Source=== | ===Source=== | ||
Revision as of 11:07, 20 September 2019
GlyRS protein equipped with a 6x HIS affinity tag
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 2989
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1737
Illegal AgeI site found at 471
Illegal AgeI site found at 2859 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 958
Design Notes
The sequence was extracted from the pET21a-GlyRS-His plasmid and expressed in the BL21 (DE3) competent E.coli strain
Codon optimization : 1509 from A to G
A second stop codon was added in compliance with iGEM standards.
Source
Sebastian Maerkl Lab at EPFL, Switzerland.
https://www.addgene.org/124110/
References
Cell-free translation reconstituted with purified components. Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, Ueda T. Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802. 10.1038/90802 PubMed 11479568
Lavickova Barbora, and Sebastian J. Maerkl. A Simple, Robust, and Low-Cost Method to Produce the PURE Cell - Free System. preprint, Synthetic Biology, 18 Sept. 2018. DOI.org (Crossref), doi:10.1101/420570.