Difference between revisions of "Part:BBa K2916003"

 
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<partinfo>BBa_K2916001 short</partinfo>
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<partinfo>BBa_K2916003 short</partinfo>
  
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Arginyl-tRNA synthetase is a fusion protein that is used in the PURE and OnePot PURE cell-free systems.  
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Glycyl-tRNA synthetase is a fusion protein that is used in the PURE and OnePot PURE cell-free systems.  
  
  
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<!-- Add more about the biology of this part here
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===Usage and Biology===
 
===Usage and Biology===
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Used in OnePot PURE
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2916001 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K2916003 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K2916001 parameters</partinfo>
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<partinfo>BBa_K2916003 parameters</partinfo>
 
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Revision as of 10:48, 20 September 2019


GlyRS protein equipped with a 6x HIS affinity tag

Glycyl-tRNA synthetase is a fusion protein that is used in the PURE and OnePot PURE cell-free systems.


Usage and Biology

Used in OnePot PURE

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2989
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1737
    Illegal AgeI site found at 471
    Illegal AgeI site found at 2859
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 958