Difference between revisions of "Part:BBa K2916000:Design"
Konstantinos (Talk | contribs) (→Design Notes) |
Konstantinos (Talk | contribs) (→Source) |
||
| Line 14: | Line 14: | ||
===Source=== | ===Source=== | ||
| − | + | Sebastian Maerkl Lab at EPFL, Switzerland. | |
| + | |||
| + | https://www.addgene.org/124104/ | ||
===References=== | ===References=== | ||
Cell-free translation reconstituted with purified components. Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, Ueda T. Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802. 10.1038/90802 PubMed 11479568 | Cell-free translation reconstituted with purified components. Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, Ueda T. Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802. 10.1038/90802 PubMed 11479568 | ||
Revision as of 13:36, 19 September 2019
ArgRS protein equipped with a 10x HIS affinity tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 76
Illegal SapI.rc site found at 1495
Design Notes
The sequence was extracted from the pQE30-His-AlaRS plasmid and expressed in the M15 E.coli strain
1512 from A to T
Source
Sebastian Maerkl Lab at EPFL, Switzerland.
https://www.addgene.org/124104/
References
Cell-free translation reconstituted with purified components. Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, Ueda T. Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802. 10.1038/90802 PubMed 11479568