Difference between revisions of "Part:BBa K2916000:Design"
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The sequence was extracted from the pQE30-His-AlaRS plasmid and expressed in the M15 E.coli strain | The sequence was extracted from the pQE30-His-AlaRS plasmid and expressed in the M15 E.coli strain | ||
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| + | 1512 from A to T | ||
===Source=== | ===Source=== | ||
Revision as of 13:34, 19 September 2019
ArgRS protein equipped with a 10x HIS affinity tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 76
Illegal SapI.rc site found at 1495
Design Notes
The sequence was extracted from the pQE30-His-AlaRS plasmid and expressed in the M15 E.coli strain
1512 from A to T
Source
http://www.addgene.org/124103/
References
Cell-free translation reconstituted with purified components. Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, Ueda T. Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802. 10.1038/90802 PubMed 11479568