Difference between revisions of "Part:BBa K2992026"

 
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<partinfo>BBa_K2992026 short</partinfo>
 
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<i>botR</i> integration module for <i>C. sporogenes</i> with native RBS for polar expression from <i>pyrD</i>
 
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===Usage and Biology===
 
===Usage and Biology===
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This parts entry represents an integration module for the expression of <i>botR</i> at the <i>pyrE</i> locus of the <i>C. sporogenes</i> genome. This module comprises the <i>botR</i> gene of<i>C. botulinum</i> [https://parts.igem.org/Part:BBa_K2992002 BBa_K2992002] coupled with its native RBS [https://parts.igem.org/Part:BBa_K2992011 BBa_K2992011] to encourage transcription from the promoter of the <i>pyrD</i> gene following integration onto the chromosome of <i>C. sporogenes</i>.  A strong clostridial terminator was included to prevent polar transcription of <i> pyrE</i> and any downstream genes on the chromosome of <i>C. sporogenes</i> [https://parts.igem.org/Part:BBa_K2992013 BBa_K2992013]. In our project we use the transcriptional regulator of neurotoxin production from <i>C. botulinum</i>, BotR, to control the regulation of our volatile reporter operons (hyperlink to comp parts) and our fluorescent reporter FAST [https://parts.igem.org/Part:BBa_K2992000 BBa_K2992000] through interaction with its own promoter sequence P<i>botrR</i> [https://parts.igem.org/Part:BBa_K2992012 BBa_k299012] and P<i>ntnH</i> [https://parts.igem.org/Part:BBa_K2992001 BBa_K2992001] whose genes are cognate members of the BotR regulon. Doing so allows us to use our surrogate host strain <i>C. sporogenes</i> as a model system for predicting botulinum neurotoxin production following food manufacture, through the detection of our chosen reporters.<br><br>
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===Characterisation===
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Data incoming
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<br><br>
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2992026 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2992026 SequenceAndFeatures</partinfo>
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===References===
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Cañadas et al., 2019 RiboCas – update
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Dupuy  and Matamouros, 2006 update
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Minton et al., 2016 Road map – update
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Raffestin et al 2009 update
  
  

Revision as of 10:37, 18 September 2019


botR integration module for C. sporogenes with native RBS for polar transcription

Usage and Biology

This parts entry represents an integration module for the expression of botR at the pyrE locus of the C. sporogenes genome. This module comprises the botR gene ofC. botulinum BBa_K2992002 coupled with its native RBS BBa_K2992011 to encourage transcription from the promoter of the pyrD gene following integration onto the chromosome of C. sporogenes. A strong clostridial terminator was included to prevent polar transcription of pyrE and any downstream genes on the chromosome of C. sporogenes BBa_K2992013. In our project we use the transcriptional regulator of neurotoxin production from C. botulinum, BotR, to control the regulation of our volatile reporter operons (hyperlink to comp parts) and our fluorescent reporter FAST BBa_K2992000 through interaction with its own promoter sequence PbotrR BBa_k299012 and PntnH BBa_K2992001 whose genes are cognate members of the BotR regulon. Doing so allows us to use our surrogate host strain C. sporogenes as a model system for predicting botulinum neurotoxin production following food manufacture, through the detection of our chosen reporters.

Characterisation

Data incoming

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Cañadas et al., 2019 RiboCas – update Dupuy and Matamouros, 2006 update Minton et al., 2016 Road map – update Raffestin et al 2009 update