Difference between revisions of "Part:BBa K3168002:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | A flexible (GGS)5 linker is located in front of the large bit to enable the formation of fusion proteins. A strep tag is also included at the end for protein purification. | + | A flexible (GGS)<sub>5</sub> linker is located in front of the large bit to enable the formation of fusion proteins. A strep tag is also included at the end for protein purification. |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
| − | deep-sea shrimp derived luciferase | + | Split variant of deep-sea shrimp derived luciferase. Sequence was derived from (Dixon, 2016). |
===References=== | ===References=== | ||
Latest revision as of 12:52, 17 September 2019
LargeBitNanoLuc
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 526
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
A flexible (GGS)5 linker is located in front of the large bit to enable the formation of fusion proteins. A strep tag is also included at the end for protein purification.
Source
Split variant of deep-sea shrimp derived luciferase. Sequence was derived from (Dixon, 2016).
References
Dixon, A. S., Schwinn, M. K., Hall, M. P., Zimmerman, K., Otto, P., Lubben, T. H., ... & Wood, M. G. (2015). NanoLuc complementation reporter optimized for accurate measurement of protein interactions in cells. ACS chemical biology, 11(2), 400-408.
Ohmuro-Matsuyama, Y., & Ueda, H. (2019). Protein-Protein Interaction Assays Using Split-NanoLuc. In Bioluminescence. IntechOpen.