Difference between revisions of "Part:BBa K3168001:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Designed for bacterial expression. | + | Designed cysteine free and for bacterial expression. |
===Source=== | ===Source=== | ||
Latest revision as of 12:48, 17 September 2019
dCas9-CP1041
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2143
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 261
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Designed cysteine free and for bacterial expression.
Source
Mutated variant of S. pyogenes derived Cas9, which was synthesised in gBlocks by IDT. The sequence was based on Addgene plasmid #119808.
References
Oakes, B. L., Fellmann, C., Rishi, H., Taylor, K. L., Ren, S. M., Nadler, D. C., ... & Savage, D. F. (2019). CRISPR-Cas9 circular permutants as programmable scaffolds for genome modification. Cell, 176(1-2), 254-267.
Park, J. J., Dempewolf, E., Zhang, W., & Wang, Z. Y. (2017). RNA-guided transcriptional activation via CRISPR/dCas9 mimics overexpression phenotypes in Arabidopsis. PloS one, 12(6), e0179410.
Ran, F. A., Hsu, P. D., Wright, J., Agarwala, V., Scott, D. A., & Zhang, F. (2013). Genome engineering using the CRISPR-Cas9 system. Nature protocols, 8(11), 2281.
Sternberg, S. H., LaFrance, B., Kaplan, M., & Doudna, J. A. (2015). Conformational control of DNA target cleavage by CRISPR–Cas9. Nature, 527(7576), 110.