Difference between revisions of "Part:BBa K2992017"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | Ferredoxin is an iron-sulfur protein involved in central redox reactions occurring during the general metabolism of Clostridia. 5’-UTR and RBS region for fdx is found naturally downstream of Pfdx and upstream of the start codon. In our project we use the regulatory region for the fdx gene comprising Pfdx-t14c ( | + | Ferredoxin is an iron-sulfur protein involved in central redox reactions occurring during the general metabolism of Clostridia. 5’-UTR and RBS region for fdx is found naturally downstream of Pfdx and upstream of the start codon. In our project we use the regulatory region for the fdx gene comprising Pfdx-t14c ([https://parts.igem.org/Part:BBa_K2992016 BBa_K2992016]) and this entry to regulate our volatile reporter genes for predicting the production of botulinum neurotoxin following food manufacture. <br><br> |
===Characterisation=== | ===Characterisation=== | ||
Revision as of 13:11, 16 September 2019
5-UTR containing RBS for fdx gene from C. sporogenes
5’-UTR containing RBS for fdx gene from C. sporogenes
Usage and Biology
Ferredoxin is an iron-sulfur protein involved in central redox reactions occurring during the general metabolism of Clostridia. 5’-UTR and RBS region for fdx is found naturally downstream of Pfdx and upstream of the start codon. In our project we use the regulatory region for the fdx gene comprising Pfdx-t14c (BBa_K2992016) and this entry to regulate our volatile reporter genes for predicting the production of botulinum neurotoxin following food manufacture.
Characterisation
Data incoming
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Cañadas et al., 2019 RiboCas - update Minton et al., 2016 Road map - update