Difference between revisions of "Part:BBa K2992014"
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===Usage and Biology===
 
===Usage and Biology===
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The native 5’-UTR containing the RBS is found naturally upstream of <i>botR</i> in <i>C. botulinum</i>. BotR is an alternative sigma factor involved in the positive regulation of botulinum neurotoxin and associated genes. In our project, we use P<i>botR</i> to drive the expression of <i>botR</i>  (add hyperlink) which in turn, regulates the production of our volatile reporter genes which we have placed under the control of neurotoxin and neurotoxin-associated promoters (add hyperlinks). Doing so allows us to link volatile reporter production with neurotoxin production following food manufacturing processes.            <br><br>
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The native 5’-UTR containing the RBS is found naturally upstream of <i>botR</i> in <i>C. botulinum</i>. BotR is an alternative sigma factor involved in the positive regulation of botulinum neurotoxin and associated genes. In our project, we use P<i>botR</i> to drive the expression of <i>botR</i>  ([https://parts.igem.org/Part:BBa_K2992002 BBa_K2992002) which in turn, regulates the production of our volatile reporter genes which we have placed under the control of neurotoxin and neurotoxin-associated promoters (add hyperlinks). Doing so allows us to link volatile reporter production with neurotoxin production following food manufacturing processes.            <br><br>
  
 
===Characterisation===
 
===Characterisation===

Revision as of 12:26, 16 September 2019


5-UTR containing RBS for botR gene from C. botulinum

5’-UTR containing RBS region for botR gene in C. botulinum


Usage and Biology

The native 5’-UTR containing the RBS is found naturally upstream of botR in C. botulinum. BotR is an alternative sigma factor involved in the positive regulation of botulinum neurotoxin and associated genes. In our project, we use PbotR to drive the expression of botR ([https://parts.igem.org/Part:BBa_K2992002 BBa_K2992002) which in turn, regulates the production of our volatile reporter genes which we have placed under the control of neurotoxin and neurotoxin-associated promoters (add hyperlinks). Doing so allows us to link volatile reporter production with neurotoxin production following food manufacturing processes.

Characterisation

Data incoming

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Raffestin et al., 2009 to be updated.