Difference between revisions of "Part:BBa K2933110:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
None.
+
CPS-1 is codon optimized for BL21(DE3) E. coli.
 
+
 
+
  
 
===Source===
 
===Source===

Latest revision as of 15:41, 14 September 2019


GST+Linker+CPS-1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1235
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1235
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1517
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1235
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1235
    Illegal AgeI site found at 1136
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85


Design Notes

CPS-1 is codon optimized for BL21(DE3) E. coli.

Source

NCBI.

References