Difference between revisions of "Part:BBa K3145002:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | There was an illegal XbaI cut site in between our T7 promoter and RBS region that was corrected through site-directed mutagenesis. | + | There was an illegal XbaI cut site in between our T7 promoter and RBS region in our pY71-sfGFP plasmid that was corrected through site-directed mutagenesis. |
Revision as of 03:47, 12 September 2019
J18912-sfGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 20
Design Notes
There was an illegal XbaI cut site in between our T7 promoter and RBS region in our pY71-sfGFP plasmid that was corrected through site-directed mutagenesis.
Source
N/A