Difference between revisions of "Part:BBa K3056001"
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Revision as of 23:35, 10 September 2019
Anti-THC antibody fragment (ScFv), bound to EGFP at the C-terminal
Part encodes for an antibody fragment designed to detect delta-9-tetrahydrocannabinol (THC). The antibody fragment is linked to EGFP via a short GSGSGS linker on the C-terminal. The transcript is codon optimized for expression in E. coli. Additionally, an OmpA sequence proceeds the transcript, which serves as a per-plasmic localization signal. To function properly, the protein needs to form disulfide bonds, which it can only do in the oxidizing environment of the peri-plasmic environment. However, for best expression, we recommend Rosetta-gami2 for expression, which contains an oxidizing cytoplasmic environment.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 691
Illegal AgeI site found at 1380 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 820