Difference between revisions of "Part:BBa K2992008"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | Thiolases are core metabolic enzymes involved in the melavonate pathway responsible for the conversion of acetyl-CoA to acetoacetyl-CoA. Results from our genome scale modeling suggested that exogenous thiolase was required for the production of acetone in our chosen volatile reporter organism <i>C. sporogenes</i>. Thiolase was therefore included in our acetone production pathway constructs in order to a volatile reporter for the prediction of botulinum toxin production following food manufacturing processes | + | Thiolases are core metabolic enzymes involved in the melavonate pathway responsible for the conversion of acetyl-CoA to acetoacetyl-CoA. Results from our genome scale modeling suggested that exogenous thiolase was required for the production of acetone in our chosen volatile reporter organism <i>C. sporogenes</i>. Thiolase was therefore included in our acetone production pathway constructs in order to a volatile reporter for the prediction of botulinum toxin production following food manufacturing processes. <br><br> |
===Characterisation=== | ===Characterisation=== | ||
Revision as of 14:37, 9 September 2019
Thiolase gene thl from C. acetobutylicum.
The thiolase gene encodes an acetyl-coenzyme A acetyltransferases from C. acetobutylicum.
Usage and Biology
Thiolases are core metabolic enzymes involved in the melavonate pathway responsible for the conversion of acetyl-CoA to acetoacetyl-CoA. Results from our genome scale modeling suggested that exogenous thiolase was required for the production of acetone in our chosen volatile reporter organism C. sporogenes. Thiolase was therefore included in our acetone production pathway constructs in order to a volatile reporter for the prediction of botulinum toxin production following food manufacturing processes.
Characterisation
Data incoming.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 355
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 355
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 355
- 1000COMPATIBLE WITH RFC[1000]
References
Incoming.