Difference between revisions of "Part:BBa K3257043"
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<span class='h3bb'>'''Sequence and Features'''</span>
 
<span class='h3bb'>'''Sequence and Features'''</span>
 
<partinfo>BBa_K3257043 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3257043 SequenceAndFeatures</partinfo>
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===References===
 
===References===

Revision as of 11:36, 9 September 2019

Lower Fidelity MMLV gag-pol Polyprotein

This part is a mutant of MMLV Reverse Transcriptase and previous research shows that the specific amino acid mutation Y586F further improves its error-prone nature of MMLV reverse transcriptase by about 5-fold in a single replication cycle.[1]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 174
    Illegal BglII site found at 1286
    Illegal BglII site found at 2592
    Illegal BglII site found at 3824
    Illegal BamHI site found at 2609
    Illegal BamHI site found at 2915
    Illegal XhoI site found at 940
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1825
    Illegal AgeI site found at 3396
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] Wen-Hui Zhang, Evguenia S. Svarovskaia, Rebekah Barr, and Vinay K. Pathak. Y586F mutation in murine leukemia virus reverse transcriptase decreases fidelity of DNA synthesis in regions associated with adenine–thymine tracts[J]. Proc Natl Acad Sci U S A. 2002 Jul 23; 99(15): 10090–10095